Comparision of ERIC-PCR, OUT- PCR and Spoligotyping Methods to Diagnose of Cross-Contamination with Mycobacterium tuberculosis

The culture of Mycobacterium tuberculosis from clinical specimens is considered to be the gold standard for the diagnosis of tuberculosis. However, false-positive cultures can occur due to cross contamination. Molecular typing methods are a useful tool for quick diagnosis of false-positive culture. In this study, we aimed to comperative evaluation of false-positive culture results by using ERIC-PCR, OUT PCR and spoligotyping methods. In the presentinvestigation, we evaluated a total of 29 culture isolates obtained from five groups samples processed on the same day during 5 different time periods. By using spoligotyping, 5 spoligotype family including LAM9, LAM7-TUR, T and an unidentified spoligotype were identified according to SITVIT2 database. With the OUT-PCR, 4 different band pattern was determined at Nusieve agarose jel electrophoresis. For ERIC-PCR method, 2-3 band pattern were obtained at Nusive agarose jel and 6-7 band pattern were determined at polyacriylamide gel electrophoresis. All theisolates were collected in two groups by ERIC-PCR, but this created groups were not efficient to determine the degree of separation between isolates. In the study, spoligotyping and OUT-PCR groups modeling were compared with each other revealed compliance rate of 96.55% between the two tests. The application of OUT-PCR has been serve as a rapid and efficient method to investigate cases of possible cross-contamination with M. tuberculosis.